Urease is amidohydrolase enzyme (EC: 3.5.1.5) splits urea into carbon dioxide and ammonia. The enzyme was purified from fenugreek (Trigonella foenum-graecum) leaves using ammonium sulphate precipitation (80%), phenyl sepharose, hydroxyapatite and sephadex G-200. The specific activity of the purified urease from sephadex G-200 was 210 U per mgprotein with 6.6 % yield. The final fold of purification was 677. The optimal pH of the purified urease was 8.0 whereas the optimal temperature was 40°C. The purified urease exhibited appreciable thermal stability at 60 °C particularly in presence of bovine serum albumin (BSA). Also, the enzyme exhibited appreciable storage stability at 25°C.
(2023). Purification and characterization of urease from leaves of Trigonella foenum-graecum. Mansoura Journal of Chemistry, 62(4), 70-75. doi: 10.21608/mjcc.2023.412061
MLA
. "Purification and characterization of urease from leaves of Trigonella foenum-graecum", Mansoura Journal of Chemistry, 62, 4, 2023, 70-75. doi: 10.21608/mjcc.2023.412061
HARVARD
(2023). 'Purification and characterization of urease from leaves of Trigonella foenum-graecum', Mansoura Journal of Chemistry, 62(4), pp. 70-75. doi: 10.21608/mjcc.2023.412061
VANCOUVER
Purification and characterization of urease from leaves of Trigonella foenum-graecum. Mansoura Journal of Chemistry, 2023; 62(4): 70-75. doi: 10.21608/mjcc.2023.412061
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